the ayurvedic pharmacopoeia of india - ccras ( pdfdrive ) (830801), страница 14
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From this stock solution pipette out aliquots of 2 to 6 ml and make up the volumeto 5 ml in volumetric flasks with methanol. Apply 10 ml of each standard solution(corresponding to 320 to 960 ng of vasicine) on TLC plate. Develop the plate to adistance of 8 cm using ethyl acetate : methanol : ammonia (8 : 2 : 0.2) as mobile phase.After development, dry the plate and scan in TLC scanner at a wavelength of 298 nm.Note the peak area under the curve for a peak corresponding to vasicine and prepare thecalibration curve by plotting peak area vs amount of vasicine.Extract accurately weighed about 5 g of Vāsāvaleha in methanol (25 ml x 5). Filter theextract, pool, concentrate and adjust the volume to 25 ml. Apply 10 ml of test solution onTLC plate and develop, dry and scan the plate as described in the preceeding paragraphfor calibration curve of vasicine.
Calculate the amount of vasicine in the test solutionfrom the calibration curve of vasicine.Estimation of piperine: Dissolve 5 mg of piperine in 100 ml of methanol. From this stocksolution, pipette out 0.8 to 4.8 ml aliquots into 10 ml volumetric flasks and make up thevolume with methanol to prepare standard solutions of 4 to 24 µg / ml. Apply 10 ml ofeach standard solution (corresponding to 40 to 240 ng) on TLC plate and develop theplate to a distance of 8 cm using dichloromethane : ethyl acetate (7.5 : 1) as mobilephase.
After development, dry the plate and scan in TLC scanner at a wavelength of 337nm. Note the peak area under the curve for a peak corresponding to piperine and preparethe calibration curve by plotting peak area vs amount of piperine.Extract accurately weighed about 5 g of Vāsāvaleha with ethyl acetate (25 ml x 5). Filterthe extract, pool, concentrate and adjust the volume to 25 ml in a volumetric flask. Apply10 ml of test solution on TLC plate and develop, dry and scan the plate as described in thepreceding paragraph for calibration curve of piperine. Calculate the amount of piperine inthe test solution from the calibration curve of piperine.Other requirements:Microbial limits:Aflatoxins:Appendix 2.4.Appendix 2.7.Storage: Store in a cool place in tightly closed containers, protected from light andmoisture.Therapeutic uses: Kāsa (cough); Śvāsa (Dyspnoea); Jvara (Fever); Raktapitta(bleeding disorders); Rājayak¾mā (Tuberculosis); Pārśvaśūla (intercostal neuralgia andpleurodynia); H¨tśūla (Angina pectoris).Dose: 12 g daily in divided doses.Anupāna: Milk, Water.VYĀGHRĪ HARĪTAKĪ(AFI, Part-II, 3:6)Definition:Vyāghrī Harītakī is a semisolid preparation made with the ingredients given in theFormulation composition.Formulation composition:1.2.3.4.5.6.7.8.9.10.11.12.Ka´°akārī APIJala API for decoctionreduced toHarītakī APIGu²a APIŚu´°hī APIMarica APIPippalī APITvak APIPatra (Tvakpatra API)Elā (Sūk¾mailā API)Nāgakeśara APIPu¾parasa (Madhu API)Solanum surattenseWaterPl.Terminalia chebulaJaggeryZingiber officinalePiper nigrumPiper longumCinnamomum zeylanicumCinnamomum tamalaElettaria cardamomumMesua ferreaHoneyP.
(100 in No.)Rz.Fr.Fr.St. Bk.Lf.Sd.Stmn.4.8 kg12.9 l3.07 l1.2 kg4.8 kg96 g96 g96 g48 g48 g48 g48 g288 gMethod of preparation:Take raw material of Pharmacopoeial quality.Wash, dry and grind ingredient number 1 (Kvātha Dravya) of the formulationcomposition and pass through sieve number 44 to obtain a coarse powder.Clean, dry and powder the ingredients number 5 to 11(Prak¾epa Dravya) of theformulation composition and pass through sieve number 85 to obtain a fine powder.Clean, dry the ingredient number 3 of the formulation composition and make in to smallpieces by removing seeds.
Tie the pieces of Harītakī in a muslin cloth to prepare a po°°alī.Add specified amount of water to the Kvātha Dravya and suspend the pottali containingpieces of Harītakī in to the vessel. Heat, reduce the volume to one fourth and filterthrough muslin cloth to obtain Kvātha.Collect the soft pieces of Harītakī from the po°°ali (bundle) and prepare fine paste.Add jaggery to the Kvātha, boil to dissolve and later filter through muslin cloth. Add finepaste of Harītakī, subject to gentle boiling and stir continuously during the process.Continue heating till the preparation reaches the consistency of leha confirmed by theformation of soft ball that does not disperse in water.
Stop heating.Cool to room temp and add powdered Prak¾epa Dravya and honey.Mix thoroughly to prepare a homogeneous mass.Pack it in tightly closed containers to protect from light and moisture.Description:A blackish brown, semisolid sticky paste with bitter and astringent taste and spicypleasant odour.Identification:Microscopy:Take about 5 g of the Avaleha and wash it with warm water till guda and honey areremoved.
Collect the sediment. Clarify a small amount of residue with chloral hydratesolution, wash in cold water, and mount in glycerin. Take a few mg, add iodine solutionwater, and mount in glycerin. Observe following character in different mounts.Fragments of hypodermis in surface view, stone cells varying in sizes, shapes andthickness, mostly present in groups interspersed among parenchyma (Marica); fragmentsof fibres with very narrow lumen, not over 600 μ long and not over 45 μ broad;parenchyma cells containing minute acicular crystal of calcium oxalate, stone cellsvarying shape and size, smaller ones somewhat rectangular; oil cells present (Tvak);groups of slightly wavy parenchymatous cells, each cell containing 1 to 3 rosette crystalsof calcium oxalate, groups of perisperm cells bulbous in shape packed with starch grainswhich also shows in middle tiny prismatic crystals of calcium oxalate; epidermal andhypodermal cells crossing each other at right angle (Sūkşmailā); groups ofparenchymatous cells, densely packed with starch grains, isolated starch grains, simple,oval to rod shaped upto 75 μ in length, hilum eccentric, lamellae distinct; yellow colouredoleo resin cells, non-lignified, septate fibres some of them bearing marks of adjacent cellspressing against them (Śu´°hī); stone cells with broad lumen in groups of 2 to 8(Pippalī); crushed pieces of anther lobes containing pollen grains, each tricolporatemeasuring upto 55 μ in dia., groups of epidermal cells of anther lobe (Nāgakeśara);groups of angular epidermal parenchytamous cells with sunken stomata, oil cells and oilglobules seen, unicellular and bicellular trichomes (Tejapatra).Thin layer chromatography:Extract 5 g of sample with n-hexane (25 ml x 3) under reflux on a water bath for 30 min,filter, concentrate to 10 ml and carry out thin layer chromatography.
Apply 10 µl of theextract on TLC plate. Develop the plate to a distance of 8 cm using tolune : ethyl acetate(8 : 2) as mobile phase. After development, allow the plate to dry in air and examineunder ultra violet light (366 nm). It shows major spots at Rf 0.28 (blue), 0.43 and 0.58(faint blue). Spray the plate with anisaldehyde- sulphuric acid reagent followed byheating at 1100 about for 10 min. It shows major spots at Rf 0.21 (green), 0.43 (blue) and0.58 (brown) under visible light.Physico-chemical parameters:Loss on drying:2.2.10.Total ash:2.2.3.Acid-insoluble ash:2.2.4.Sulphated Ash:2.2.6.Alcohol-soluble extractive:2.2.7.Water-soluble extractive:2.2.8.pH of 1% aqueous solution :Not more than 23.0 per cent,AppendixNot more than 4.0 per cent,AppendixNot more than 0.15 per cent,AppendixNot more than 0.41 per cent,AppendixNot less than 20.0 per cent,AppendixNot less than 68.7 per cent,Appendix5.5 and 5.6,Appendix 3.3.Other requirements:Microbial limits:Aflatoxins:Appendix 2.4.Appendix 2.7.Storage: Store in a cool place in tightly closed containers, protected from light andmoisture.Therapeutic uses: Kāsa (cough); Pratiśyāya (Coryza); Śvāsa (Asthma); Svaraksaya(aphasia); Pīnasa (Chronic rhinitis / Sinusitis); Rājayak¾mā (Tuberculosis).Dose: 5 to 15 g.Anupāna: Water, Milk.CŪR³AGeneral Descripition:Drugs according to the formulation composition of the particular cūr´a arecollected, dried, powdered individually and passed through sieve number 85 to prepare afine powder.
They are mixed in the specified proportion and stored in well closedcontainer.The term cūr´a may be applied to the powder prepared by a single drug or acombination of more drugs.Raja and K¾oda are the synonyms for cūr´a. Cūr´as may be of plant origin, ormixed with other ingredients. The following points are to be noted.If metals / minerals are used, prepare bhasma or sindura of the minerals unlessotherwise mentioned.In cases where pārada and gandhaka are mentioned, prepare Kajjalī and add otherdrugs, one by one, according to the formula.In general the aromatic drugs like Hi¬gū [Asafoetida] etc. should be fried beforethey are converted to fine powders.Specific care should be taken in case of Salts and Sugars. Formulations withhygroscopic components should not usually be prepared during rainy seasons.
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