Van Eyk, Dunn - Proteomic and Genomic Analysis of Cardiovascular Disease - 2003 (522919), страница 59
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It is expected that defining the activity ofeach individual element and its relationship to other elements in that system willlead to a more integrated and global understanding of how those elements function together in a biological network. The genomics field has provided examplesthat support this concept through the application of cDNA microarray technology,e.g.
[1, 2]. Large-scale gene expression profiling (via cDNA microarray technology)has revealed that co-regulated genes tend to cluster into distinct functional categories, facilitating the classification of uncharacterized genes to discrete functionsand specific pathways [1, 3–6]. This technology has also revealed that distinct cellular phenotypes share similar mRNA expression profiles, again suggesting thatuncovering gene expression patterns will lead to a better understanding of thegenes that give rise or contribute to the maintenance of a particular cellular state(e.g. [7–16].
However, the underlying premise behind the application of DNA microarray technology is that changes in mRNA abundance directly translate tochanges in the abundance of the proteins encoded by those mRNAs. Proteins arethe molecular effectors that carry out essentially all biochemical activities of thecell. Therefore, mRNA profiling represent an indirect assay from which proteinabundance and activity are inferred. Proteomics is the discipline that attempts tosystematically and quantitatively analyze the proteins expressed in a cell or tissueand to therefore provide a more direct indication of the functional state of thecell.
Mass spectrometry (MS) is a critical component of essentially any currentproteome analysis technology and has had a significant impact on the growth anddevelopment of the proteomic field. In particular, protein identification and quantification are most commonly achieved by the application of one of a variety ofMS technologies [17]. In this chapter we will highlight new developments in protein reactive chemistries (e.g. isotope coded affinity tags ICATTM reagents) thatwhen used in combined with MS allow for the global identification and quantification of the proteins present in two or more complex samples such as those reProteomic and Genomic Analysis of Cardiovascular Disease.Edited by Jennifer E. van Eyk, Michael J. DunnCopyright © 2003 WILEY-VCH Verlag GmbH & Co.
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