Van Eyk, Dunn - Proteomic and Genomic Analysis of Cardiovascular Disease - 2003 (522919), страница 55
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Then, onceformed, the ionised particles are accelerated into the analyser, where they are separated and mass analysed before being detected electronically at the detector.This chapter will provide the reader with an overview of the different ionisationmethods and introduce various mass analysers and instrument configurations.Strategies for protein identification and characterisation will be explained, illustrated with examples to show how these powerful analytical tools can be appliedto the study of proteins and peptides.12.1Ionisation ProcessesIn order that ions can be analysed they have to be created and introduced into thegaseous phase. There are various methods of ionisation including electron impact(EI), chemical ionisation (CI), plasma desorption (PD) [1] and fast atom bombardment (FAB) [2].
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