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- Vol. 53. - P. 853–857.97ПРИЛОЖЕНИЕПриложение 1Распределение всех филотипов (доминантные и минорные) по всем горизонтам водного столба и истока реки, а также трём праймернымсистемамБлижайший вид из GenBankСходство,%1,3 мv3-v5ПрокариотыActinobacteriaCandidatus Planktophila limnetica (FJ428831.1)Клад acI-AVIКлад acI-AIVНеопределённый клад подгруппы acI-AMycobacterium pyrenivorans (NR 028970)Ilumatobacter fluminis (NR_041633)Solirubrobacter soli (AB245334)α-proteobacteriaRoseomonas frigidaquae (NR_044455.1)Rhodovarius lipocyclicus (NR_025629)Ketogulonicigenium vulgare (AF136848)Mesorhizobium loti (NR_074162)β-proteobacteriaLimnohabitans planktonicus (FM165535)Polaromonas vacuolata (NR_025958)Herbaspirillum huttiense (EU046556)δ-proteobacteriaDesulfomicrobium hypogeium (AF132738)BacteroidetesChitinophaga sancti (AB078068)Sediminibacterium salmoneum (NR_044197)96,596,296,898,893,987*261(8)16(5)97,394,197,798,0110(3)v4-v8Ген 16S26(10)56(20)11214(4)28(8)121(6)10(3)14(4)21(4)42(8)23(18)125(20)17(13)5(4)Исток реки2м367 мv4-v8Ген 16S12(9)7(4)v3-v525(6)42(10)11(6)v4-v8145(9)112114(3)115(3)9(5)11116(3)99,197,399,713(5)12(3)1183*95,499,0ГоризонтыВодный столб100 м200 мКлоновые библиотеки, %v3-v5v4-v8v3-v5 v4-v8 v3-v54(3)10(3)29(5)7(4)210(4)10(2)98Pedobacter composti (AB267720)Flavobacterium frigidarium (AB455257)Adhaeribacter aquaticus (AJ626894PlanctomycetesBlastopirellula marina (NR_029226)Singulisphaera rosea (FN391026)Schlesneria paludicola (NR_042466.1)Algisphaera agarilytica (AB845176)Phycisphaera mikrensis (AB474364)Phycisphaera mikrensis (AB474364)Gemmata obscuriglobus (X85248)Candidatus Nostocoida acidiphila_JQ067914Candidate division OD1Uncult.
bact. (AY922093)Uncult. bact. (FJ482182)VerrucomicrobiaChthoniobacter flavus (AY388649)Pedosphaera parvula (AY960777)Prosthecobacter vanneervenii (NR_026022)Candidatus Xiphinematobacter rivesi (AF217461)Opitutus terrae (AJ229246)ЭукариотыViridiplantae, зелёные водорослиPedinomonas minor (FJ968740) хпДНКMychonastes huancayensi (GQ477050)StramenopilesXanthophyta (мтДНК)Nannochloropsis oceanica (KC568462)Nannochloropsis limnetica (KC568459)Dictyochophyceae (хпДНК)Mesopedinella arctica (AY702158)Chrysophyceae (хпДНК)Ochromonas distigma (AY702136)96,697,887*12188*94,690*85*83,7*81*82*9523(9)10(4)8(3)7(2)11(2)210(2)6(5)1196,586*91,491,393,312122219(6)84(32)74*99,918(3)193,52225(9)2131(9)92,9– процент клонов в библиотеке.В скобках - число клонов в библиотеке* - неидентифицированный филотипСерым фоном выделены общие для горизонтов филотипы.6(5)128(8)Примечание – число филотипов в каждом горизонте дано без учета НА-филотипов110(2)7(4)1190,9*95,8*88*99,617(2)15(3)54(21)12(3)5(3)10(3)15(3)10(4)120(4)99Приложение 2Значимые находки, имеющие отношение к холодным местообитаниямКлон/штаммБлижайший таксон в GeneBank(номер)Сходство,%ИсточникссылкаФизиологическаягруппа99,6осадки СеверногоЛедовитого океана;придонные маты,Сухие Долины,Антарктида(Yuan et al., 2014)(Rojas et al., 2009)ПТ ХО100Осадки ледника,Шпицберген(Reddy et al., 2009)ПФ ХО99,1Пресноводныйрезервуар, Чехия(Kasalický et al., 2010)ПТ ХО99,0Осадки резервуара,Пекин(Qu and Yuan, 2008)МФ ХО99,6оз.
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