Анализ специфичности расщепления ДНК ультразвуком (1102352), страница 14
Текст из файла (страница 14)
Характер изменения частоты расщепления фосфодиэфирных связей от их положенияв цепи фрагмента, а также специфика зависимости общего уровня расщепления ДНК отфизико-химическихпараметровоблучаемогорастворасвидетельствуютомеханохимической природе наблюдаемых разрывов в фрагментах ДНК под действиемультразвука.3. Развит подход к моделированию расщепления фрагментов ДНК под действиемкавитационных эффектов в облучаемом растворе, на основе которого показано, чтовозникающие вблизи кавитационных пузырьков высокие градиенты скорости теченияжидкости (порядка 108 с-1) способны приводить к разрыву фрагментов ДНК длиной внесколько сотен нуклеотидных пар.4. Предложена модель, качественно описывающая явление контекстной специфичностирасщепления ДНКультразвуком.
В соответствии с разработанной моделью,наблюдаемое увеличение частоты расщепления фосфодиэфирной связи, примыкающейкдезоксицитидину,связаносособенностьюконформационнойдинамикидизоксирибозы этого нуклеотида.5. При помощи полученных статистических даных по ультразвуковому расщеплениюДНК показано, что в промоторных областях генов MYC- кластера человека, имеетместо тенденция к уменьшению ультразвукового расщепления по сравнению скодирующими областями.84ЛитератураAbeel T., Saeys Y., Bonnet E., Rouzé P., Van de Peer Y.
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