Van Eyk, Dunn - Proteomic and Genomic Analysis of Cardiovascular Disease - 2003 (522919), страница 34
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This visualization allowedus to determine those changes that were in common across patients that mightbe implicated in the disease process. In addition, we clustered the data across allthe apoptotic genes on the chip (~250 genes). This approach indicated an obviouschange in specific apoptotic signaling pathways [56].
Although in the microarrayanalysis many of the gene changes in this pathway were not statistically significant, we were able to observe statistically significant differences in expression ofmany of these genes using RT-PCR. Thus clustering of all the genes in a pathwaycan provide insights that might not be available if only a small fraction of thegenes are included. However, with this approach it is essential to confirm changesin gene expression. After identification of a pathway by microarray data, one canthen determine whether these changes occur in additional hearts. This approachuses microarray as an unbiased screening tool to identify pathways, but then confirms the importance of these pathways using other methods in a larger numberof hearts.
This type of approach may have application for identifying pathwaysthat are important in cardioprotection.6.8 References6.7SummaryGene profiling by microarray is a powerful new technology that promises to provide new insights into genes involved in cardioprotection. At present, we havemuch to learn regarding gene expression and cardioprotection.
Do all or mostmodels of cardioprotection alter expression of a few common genes or are cardioprotective genes as diverse as cardioprotective agents? We know that genetic background or context can modify cardioprotection, but we do not understand this atthe level of specific genes. We also know that depending on the level of expression a gene, such as PKC-e can be cardioprotective or can induce hypertrophy.Gene profiling is an ideal technique to address these complex issues.6.8References123456Murry C, Jennings R, Reimer K.
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