the ayurvedic pharmacopoeia of india - ccras ( pdfdrive ), страница 9

From this stock solution, prepare standard solutions of 15 to 75 mg / mlby transferring aliquots (1.5 to 7.5 ml) of stock solution to 10 ml volumetric flasks andadjusting the volume to 10 ml with methanol.Apply 10 ml of each standard solution corresponding to 150 ng to 750 ng of gallic acid ona TLC plate. Develop the plate to a distance of 8 cm using toluene : ethyl acetate : formicacid : methanol (3 : 3 : 0.8 : 0.2 ) as mobile phase. After development, dry the plate andscan in TLC scanner at wavelength of 280 nm.

Note the area under the curve for peakcorresponding to gallic acid and prepare the calibration curve by plotting peak area vsamount of gallic acid.Hydrolyze accurately weighed about 5 g of crushed modaka by refluxing with 50 ml of2N hydrochloric acid on a water-bath. Filter, add equal amount of water, transfer to aseparating funnel and extract with diethyl ether (20 ml x 4). Collect the diethyl ether layerand dry. Dissolve the residue in 25 ml of methanol.

Apply 10 ml on a TLC plate anddevelop, dry and scan the plate as described in the preceding paragraph for calibrationcurve of gallic acid. Note area under the curve for a peak corresponding to gallic acid.Calculate the amount of gallic acid in the test solution from the calibration curve of gallicacid.Other requirements:Microbial limits:Aflatoxins:Appendix 2.4.Appendix 2.7.Storage: Store in a cool place in tightly closed containers, protected from light andmoisture.Therapeutic uses: Pittārśa (anorectal growth due to pitta do¾a)Dose: 2 to 5 g daily in divided doses.Anupāna: Milk, WaterCaution: In some cases, patients may develop rashes over skin.

In such cases, applyNārikela Taila or Gh¨ta over the affected part and advise to take Nārikela internally.BILVĀDILEHA(AFI, Part-I, 3:18)Definition:Bilvādi Leha is a semisolid preparation made with the ingredients in the Formulationcomposition given below.Formulation Composition:1.2.Rt.Aegle marmelosWater3.4.5.6.7.8.Bilva API– mūlaJala API for decoctionreduced toJīr´a Gu²a (Purā´a Gu²a) APIGhana (Mustā API)Dhānya (Dhānyaka API)Jīraka (Śvetajīraka API)Trutī (Sūksmailā API)Tvak APIOld JaggeryCyperus rotundusCoriandrum sativumCuminum cyminumElettaria cardamomumCinnamomum zeylanicumRz.Fr.Fr.Sd.St. Bk.1536 g12.28 l3.072 l768 g12 g12 g12 g12 g12 g9.10.11.12.Keśara (Nāgakeśara API)Śun°ªī APIMarica APIPippalī APIMesua ferreaZingiber officinalePiper nigrumPiper longumStmn.Rz.Fr.Fr.12 g12 g12 g12 gMethod of Preparation:Take raw material of pharmacopoeial quality.Wash, dry, powder ingredient number 1 (Kvātha Dravya) of the formulation compositionand pass through sieve number 44 to obtain coarse powder.Clean, dry, powder the ingredients number 4 to 12 (Prak¾epa Dravya) of the formulationcomposition and pass through sieve number 85 to obtain fine powder.Add specified amounts of water to the Kvātha Dravya, heat, reduce to one fourth andfilter through muslin cloth.Add jaggery to the Kvātha, boil to dissolve and filter through muslin cloth.Reduce the kvātha to thicker consistency by gentle boiling and stirring continuouslyduring the process.Continue heating till the preparation attains the consistency of leha confirmed by theformation of a soft ball that doesn’t disperse in water.Remove from heat source and allow to cool to room temperature.Add fine powders of Prak¾epa Dravya, mix thoroughly to prepare a homogeneous mass.Pack it in tight closed containers to protect from light and moisture.Description:Dark brown semisolid paste with a spicy pleasant odour and sweet, astringent taste.Identification:Microscopy:Take about 5 g of avaleha and wash twice or thrice with about 20 ml of water, each timerejecting the supernatant; take a few mg of the sedimented material, stain with iodinesolution and mount in 50 per cent glycerin; clarify a few mg with chloral hydrate andmount in 50 per cent glycerin.

Observe the following characters in different mounts.Multicellular, multiseriate trichomes, fragments of vittae in surface view showingepithelial tissue elongated along the long axis of the vittae, and mesocarpic stone celllayer with cells much longer than broad (Śvetajīraka); groups of slightly wavyparenchymatous cells, each cell contains 1 to 3 rosette crystal of calcium oxalate, groupsof bulbous perisperm cells packed with starch grains which also shows in the middle tinyprismatic crystal of calcium oxalate, epidermal and hypodermal cells crossing each otherat right angle (Sūkşmailā); fragments of fibres with very narrow lumen, not over 600 μlong and not over 45 μ broad, parenchyma cells containing minute acicular crystals ofcalcium oxalate, stone cells of varying shapes and sizes with thickened walls on threesides, oil cells (Tvak); crushed pieces of anther lobes containing pollen grains, pollengrains tricolporate, measuring 25 to 55 μ in dia, unicellular and multicellular uniseriatetrichomes several showing a funneling tip or branching, groups of endothecial cells ofanther lobe (Nāgakeśara); group of parenchymatous cells, densely packed with starchgrains, isolated starch grains, simple, oval to rod shaped, measuring 15 to 70 μ in length,hilum eccentric, lamellae distinct, yellow coloured oleo resin cells, non-lignified, septatefibres some of them bearing marks of adjacent cells pressing against them, 30 to 50 μbroad, (Śu´°hī); tissue debris consisting of packed regular rows of fibre-sclereids of fairlyuniform size, and narrow scalariformed vessel showing laterally placed simpleperforation (Mustā); lignified cells, isolated or in small groups measuring 130 to 190 µ india with broad lumen, in groups of 2 to 8 (Pippalī); fragments of hypodermis in surfaceview with stone cells varying in sizes, shapes and thickness, present in groups,interspersed among parenchymatous cells (Marica); group of sclerenchymatous cells,crisscrossing each other, epidermal tissue with fairly large cells showing stomata andoctahedrons of calcium oxalate crystals, large, pentagonal, sclerenchymatous cell layer(Dhānya).Thin layer chromatography:Extract 5 g of avaleha with 75 ml of n-hexane under reflux on a water-bath for 30 min.Filter and concentrate to 10 ml and carry out the thin layer chromatography.

Apply 10 µlof the extract on TLC plate. Develop the plate to a distance of 8 cm using toluene : ethylacetate (8 : 2) as mobile phase. After development, allow the plate to dry in air andexamine under ultraviolet light (366 nm). It shows major spots at Rf 0.23, 0.30 (bothblue), 0.53 (fluorescent blue) 0.65 and 0.73 (both blue).Physico-chemical parameters:Loss on drying:2.2.10.Total ash:2.2.3.Acid-insoluble ash:2.2.4.Alcohol-soluble extractive:2.2.7.Water-soluble extractive:2.2.8.pH (1% aqueous solution) :Not more than 20.0 per cent,AppendixNot more than 2.3 per cent,AppendixNot more than 0.22 per cent,AppendixNot less than 6.8 per cent,AppendixNot less than 66.0 per cent,Appendix5.8 to 6.7,Appendix 3.3.Other requirements:Microbial limits:Aflatoxins:Appendix 2.4.Appendix 2.7.Storage: Store in a cool place in tightly closed containers, protected from light andmoisture.Therapeutic uses: Aruci (aversion to food); Agnimāndya (digestive impairment);Praseka (excessive salivation); Chardi (emesis).Dose: 6 g to be licked up 2 to 3 times in small quantities each time.CITRAKA HARĪTAKĪ(AFI, Part-I, 3:10)Definition:Citraka Harītakī is a semisolid preparation made with the ingredients in the Formulationcomposition given below:Formulation Composition:1.2.Citraka API – kvāthaĀmalakī API - kvātha3.4.Gu²ūcī API – kvāthaDaśāmūla API - kvātha(a.) Bilva API(b.) Agnimantha API(c.)(d.)(e.)(f.)(g.)(h.)(i.)(j.)5.Śyonāka APIKāśmarī (Gambhārī API)Pā°alā APIŚālapar´ī APIP¨¾nipar´ī APIŚvada¼¾trā (Gok¾ura API)B¨hatī APIKā´°akārī APIPathyā (Harītakī API)– .

cūrņa6.Guda APIPlumbago zeylanicaPhyllanthus emblica(Emblica officinalis)Tinospora cordifoliaRt.P.4.800 l4.800 lSt.4.800 l4.800 lAegle marmelosPremna mucronata(Official substitute)Oroxylum indicumGmelina arboreaStereospermum suaveolensDesmodium gangeticumUraria pictaTribulus terrestrisSolanum indicumSolanum surattenseTerminalia chebulaRt./St.

Bk.Rt./St. Bk.Rt./St. Bk.Rt./St. Bk.Rt./St. Bk.PlPlPlPlPlP.Jaggery3.07 kg4.80 kg.7.8.9.10.11.12.Śunthī APIMarica APIPippalī APITvak APIElā (Sūkşmailā API)Patra (Tejapatra API).Zingiber officinalePiper nigrumPiper longumCinnamomum zeylanicumElettaria cardamomumCinnamomum tamalaRz.Fr.Fr.St. Bk.Sd.Lf.96 g96 g96 g96 g96 g96 g13.Ksāra (Yava API)Hordeum vulgareWatersolubleAsh of Pl.24 g14.Madhu APIHoney384 g_____________________________________________________________________________Note: Stem bark of the ingredient number 4 [(a) to (e)] has been used.Method of Preparation:Wash, dry and powder the ingredients numbered 1 to 4 (Kvātha dravya) of theFormulation composition separately and pass through sieve no.

44 to obtain a coarsepowder.Dry and powder the ingredient number 5 separately and ingredients number 7 to 13(Prak¾epa dravyas) of the Formulation composition to a fine powder and pass throughsieve no. 85.Add required amount of water to the Kvātha dravya, heat, reduce to one fourth and filterthrough muslin cloth.Mix all the Kvāthas together. Add Jaggery, boil to dissolve and filter through a muslincloth.Reduce the Kvātha to a thicker consistency by gentle boiling; add cūrņa of Pathyā and stirthoroughly during the process.Add the powdered prak¾epa dravya no. 7 to 13 while hot at 500, mix thoroughly toprepare a homogeneous mass.Allow to cool to room temperature. Add honey, mix thoroughly.Pack it in tightly closed containers to protect from light and moisture.Description:Blackish brown, semisolid paste with spicy, pleasant odour and bitter-astringent taste.Identification:Microscopy:Take about 5 g of the sample, wash thoroughly and repeatedly in warm water to removeGuda and Madhu, each time rejecting the supernatant, and saving the residue withoutloss.

Take the sediment in distilled water, mix thoroughly, allow to settle, and throw offsupernatant. Take a few mg of the sediment, stain with iodine solution, mount in glycerin(50 per cent); take a few mg of sediment, clear in chloral hydrate, wash, and mount inglycerine (50 per cent).