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Shepelova I. A. Cadmium influence on the indices of nitrogen and carbohydrate metabolism in rat's organism of different ages. Manuscript.
The dissertation is submitted for the competition of scientific of candidate of biological science on speciality 03.00.04 biochemistry. National Agriculture University of Ukraine, Kiev, 2008.
The dissertation presents the results of the investigation of cadmium sulfate influence on the qualitative and quantitative composition of free amino-acids in blood, liver, kidneys, lungs, heart and spleen, the concentration of the indices of nitrogen metabolism (ammonia, creatinine, urea and total protein in the blood), the content of glycolysis metabolites (piruvate, lactate), the cycle of tricarboxylic acids (malate, oxaloacetate and б-ketoglutarate) in the liver and blood of the rats of 3-, 6 - and 18 months age.
It has been found that cadmium sulfate intoxication results both in the increase of total content of free amino-acids in the liver and kidneys, as well as in their decrease in the blood, heart, spleen and lungs of the intoxicated rats of all age groups. The most expressed changes in metabolic pool of free amino-acids of the blood and organs have been established in the rats of three months age. Cadmium intoxication caused the changes in the blood indices of rats of all age groups which characterizes the state of nitrogen metabolism (ammonium, creatinine, urea, total protein). In the blood of the intoxicated rats of 3-, 6 - and 18 months age there has been revealed the decrease in the concentration of total protein on the background of the authentic increase in the concentration of ammonia, urea and creatinine. It has been shown that under influence of cadmium sulfate in the blood and liver of the intoxicated rats of all age groups the breach of carbohydrate metabolism is observed which is characterized by the changes in the content of glycolysis metabolites and the cycle of tricarboxylic acids. It has been found that in case of cadmium sulfate intoxication, the level of the changes in biochemical indices is defined, first of all, by the age of animals and is observed mostly in rats of 3-months age.
The results of the investigation may be used for the developing new complex methods in diagnostics, precautions and corrections in pathologic changes under conditions of cadmium intoxication, considering animal's age in order to decrease cadmium toxic effect on human and animal organisms on the territories with a high level of pollution by heavy metals. The results obtained may be applied in pedagogical process in the establishments of biological, medical and veterinary fields as well as in scientific and practical research for a scientifically argumented correction of nitrogen and carbohydrate metabolism in the organism of animals which have been intoxicated by heavy metals.
Key words: cadmium sulfate, amino acids, metabolites, rats, glycolysis, cycle of tricarboxylic acids, nitrogenous metabolism, carbohydrate metabolism.
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