Apoptosis and Cell Proliferation, страница 36

No.Apoptotic DNA Ladder Kit1 835 2461 kit (20 tests)Cell Death Detection ELISAPLUS1 774 4251 920 6851 kit (96 tests)10 x 96 testsAnti-PARP1 835 238100 µlCaspase 3 Activity Assay2 012 9521 kit (96 tests)Pack SizeProducts for Measuring Apoptosis in Individual CellsCat. No.Pack SizeIn Situ Cell Death Detection Kit, Fluorescein1 684 7951 kit (50 tests)In Situ Cell Death Detection Kit, AP1 684 8091 kit (50 tests)In Situ Cell Death Detection Kit, POD1 684 8171 kit (50 tests)TUNEL Label1 767 2913 x 550 µl (30 tests)TUNEL Enzyme1 767 3052 x 50 µl (20 tests)TUNEL POD1 772 4653.5 ml (70 tests)TUNEL AP1 772 4573.5 ml (70 tests)TUNEL Dilution Buffer1 966 0062 x 10 mlDAB substrate, precipitating (POD substrate)1 718 0961 packNBT/BCIP Stock Solution (AP substrate)1 681 4518 mlFast Red Tablets (AP substrate)1 496 54920 TabletsPropidium iodide solution*1 348 63920 mlDAPI236 27610 mgEthidium bromide200 2712gAnnexin-V-FLUOS1 828 681250 testsAnnexin-V-FLUOS Staining Kit1 858 7771 kit (50 tests)Annexin-V-Biotin1 828 690250 testsAnnexin-V-Alexa™ 5681 985 485250 testsStreptavidin-Fluorescein1 055 0971 mgStreptavidin-Phycoerythrin1 428 56050 µg (1 ml)Streptavidin-POD1 089 153500 U (1 ml)Streptavidin-AP1 089 1611000 U (1 ml)M30 CytoDEATH2 140 3222 140 34950 tests250 testsAnti-Fas (CD95/Apo-1)1 922 432100 µgAnti-Fas (CD95/Apo-1) Biotin1 922 459100 testsAnti-p53 protein, pan1 413 147100 µgp53 pan ELISA1 828 7891 kit (96 tests)Anti-p53 protein pan, polyclonalunlabeledbiotin-labeledPOD-labeled1 810 9281 810 9361 810 944200 µg150 µg50 UAnti-p53 protein, mutant1 699 823100 µgAnti-bcl-2 oncoprotein, human (clone 124)1 624 9891 mlAppendixAntibodiesAnnexin-related reagentsFluorescencelabelsTUNEL related reagentsProducts for Measuring Apoptosis in Cell Populations* only sold in the US.1313Ordering GuideProducts for Measuring CytotoxicityCat.

No.Cytotoxicity Detection Kit (LDH)1 644 7931 kit (2000 tests)107 07725 mg (2.5 ml)Lactate dehydrogenaseCellular DNA Fragmentation ELISA1 585 0451 kit (500 tests)Cell Proliferation Kit I (MTT)1 465 0071 kit (2500 tests)Cell Proliferation Kit II (XTT)1 465 0151 kit (2500 tests)Cell Proliferation Reagent WST-11 644 8072500 testsProducts for Measuring Cell Proliferation in Cell PopulationsCat. No.Pack SizeCell Proliferation Kit I (MTT)1 465 0071 kit (2500 tests)Cell Proliferation Kit II (XTT)1 465 0151 kit (2500 tests)Cell Proliferation Reagent WST-11 644 8072500 testsCell Proliferation ELISA, BrdU (colorimetric)1 647 2291 kit (1000 tests)Cell Proliferation ELISA, BrdU (chemiluminescent)1 669 9151 kit (1000 tests)FixDenat1 758 7644 x 100 ml (2000 tests)BrdU Labeling and Detection Kit III1 444 6111 kit (1000 tests)Products for Measuring Cell Proliferation in Individual CellsCat.

No.In Situ Cell Proliferation Kit, FLUOS1 810 7401 kit (100 tests)In Situ Cell Proliferation Kit, AP1 758 7561 kit (100 tests)BrdU Labeling and Detection Kit I1 296 7361 kit (100 tests)BrdU Labeling and Detection Kit II1 299 9641 kit (100 tests)Anti-BrdU (clone BMC 9318)unlabeled, formalin gradefluorescein-labeled, formalin grade1 170 3761 202 69350 µg (500 µl)50 µg (500 µl)Anti-BrdU, POD-labeled(clone BMC 6H8), Fab fragments, formalin grade1 585 86015 UAnti-BrdU, AP-labeled(clone BMC 6H8) F(ab’)2 fragments, formalin grade1 758 74815 U (1 ml)Appendix5-Bromo-2’-deoxy-uridine (BrdU) tablets3132Pack SizePack Size586 06450 tabletsAnti-Casein Kinase 2a1 499 602100 µgAnti-Ki-67 (Ki-S5), formalin grade1 742 345100 µgAnti-PCNA/Cyclin (clone 19F4)unlabeledFluorescein-labeled1 170 4061 205 811200 µg200 µg (500 µl)Anti-PCNA/Cyclin, formalin grade1 486 772100 µgAnti-Topoisomerase II alpha, human (clone Ki-S1),formalin grade1 742 353100 µgAnti-Transferrin Receptor, human1 118 048200 µgOrdering GuideActinomycin C1Cat.

No.Pack Size102 00810 mgAnti-NF-kB1 697 838100 µgAnti-TNF-a, human1 198 670100 µgAnti-TNF-a-POD, human1 198 6882UCalpain inhibitor I1 086 09025 mgCalpain inhibitor II1 086 10325 mgDNase I, RNase free776 78510 000 unitsDNase I, grade I104 13220 000 unitsDNase II, grade II104 159100 mgFura-2/AM1 074 5551 mgInterleukin-1b, human, recombinant (E. coli)1 457 75610 0000 unitsInterleukin-1b, mouse recombinant (E. coli)1 444 59010 0000 unitsInterleukin-1b, human, ELISA1 600 7291 kit (96 tests)414 60310 mgIonomycin1 439 9521 mgNuclease, mung beanIonophore A 23 1871 134 4852 000 unitsNuclease S1818 330818 34810 000 units50 000 unitsNuclease S7107 92115 000 unitsNuclease P1236 2251 mgProteinase K, lyophilizate161 51925 mgStaurosporine1 055 682500 µgTNF-a, human, recombinant (E. coli)1 371 84310 µg(1 000 000 units)TNF-a, human, recombinant (yeast)1 088 93910 µg(1 000 000 units)TNF-a, mouse, recombinant (E.

coli)1 271 1565 µg(2 000 000 units)TNF-a ELISA, human1 425 9431 kit (96 tests)AppendixAdditional Products for Cell Death/Cell Proliferation Studies1333IndexAppendix3.6 Index3134AB“A0” cells 36Acridine orange 36Agents, cell death-inducing 9Alkaline elution analysis of DNA 22Annexin V-Alexa™ 568 32assays for 38binding of phosphatidylserine 31-Biotin 34-FLUOS 32-FLUOS Staining Kit 32Antibcl-2 oncoprotein 44BrdU 93cell cycle antigens 97DNA 13Fas 41Fas-Biotin 41Fluorescein 35Ki-67 98Ki-S5 98p53 46PARP 20PCNA 98Topoisomerase II 98Antibody for flow cytometric assay, seeAnti-fluoresceinAntibody to, see AntiAPO-1 41Apopain 115Apoptosisassays for cell populations 8assays for individual cells 24biochemical characteristics of 23definition of 2difference between cytotoxicity and 50difference between necrosis and 3hallmark of 8inducers of 9morphological characteristics of 3overview of 2pathways 4proteases, role of 16simultaneous detection of necrosisand 13surface morphology changes during 5Apoptotic DNA Ladder Kit 11Aspartate at proteolysis site 16bad gene 115bax 115BCIP 30bcl-2 gene 115bcl-xL gene 15bcl-xS gene 15“Beads on a string” 9Bisbenzimidazole dye, see Hoechst dyeBrdULabeling and Detection Kit I 87Labeling and Detection Kit II 87Labeling and Detection Kit III 79labeling of DNA 86incorporation assay 86release assay 60Bromodeoxyuridine, see BrdUCCAM, see CampothecinCampothecin 13, 28, 33Caspases 16Caspase 3 Activity Assay 17CD95 41Ced-3 115ced-9 gene 115Cell cycle-associated antigens 97Cell cycle, overview of 65Cell deathaccidental 2and cytotoxicity 2programmed 2Cell Death Detection ELISAPLUS 13Cell-mediated cytotoxicity 50Cell proliferationassays for cell populations 70assays for individual cells 86assays that use tetrazolium salts 113ELISA, BrdU (chemiluminescent) 81ELISA, BrdU (colorimetric) 81Kit I (MTT) 73Kit II (XTT) 74overview of 64Reagent WST-I 75Cell viability assays, see Cell proliferationassaysCellularDNA Fragmentation ELISA 54Ceramide, role in apoptosis 115Chemiluminescent cell proliferationELISA 81IndexDDAB substrate 30Damage/leakage of plasma membrane,assays for 36DAPI 36Deoxynucleotidyltransferase, terminal 25Dexamethasone 26DNA cleavage, see DNA fragmentationDNA fragmentationduring apoptosis 5, 22DNA fragments, histone-associated 10DNA end labeling 25DNA ladderappearance of 9assay for 11size of fragments 9DNA polymerase 25DNA synthesisassays 77Dyeexclusion assays 36uptake 37EEffector cells for inducing cell death 56ELISAkits 13, 79, 81End labeling of DNA 25Epidermis tissue, in vivo labeling of 89Ethidium bromide 36Exclusion assays, see Dye exclusion assaysFFADD 116False positive, TUNEL 105fasligand 40receptor 40Fast red 30FIENA 17FixDenat 84Fluorochrome staining assays formeasuring DNA loss 36Flow cytometric techniques, kits for, seeAnnexin V-FLUOS Staining Kit 32In situ Cell Death Detection Kit,Fluorescein 27In situ Cell Proliferation Kit,FLUOS 88Flow cytometric measurementof Annexin V-stained cells 32of apoptosis 24of BrdU label 89of cell cycle position 95of ISNT method 25of normal and apoptotic cells 33, 36of peripheral blood lymphocytes 26of total DNA 89of TUNEL method 28Flow cytometryantibodies useful for 93, 98assays for apoptotic cells 27, 32Formazaninsoluble 72soluble 72fos gene 117Fragmentation of DNA 22GGlucocorticoid receptor 117Granzyme 117HHallmark of apoptosis 8HeLa cells 89Histone-associated DNA fragments 10Hoechst dye 36Homeostasis, loss during cell death 3AppendixChromatin aggregation 3c-jun gene 115c-myc gene 115Colon, proliferating cells in 91Colorimetric assaysfor cytotoxicity 52, 54for proliferation 81CPP32 115Crm A 115Cr release assay, radioactive 60Cyclin 65Cysteine proteases 5Cytotoxic T cells 2Cytotoxicityassays 52, 54cell-mediated 50definition of 50Detection Kit (LDH) 52effectors of 50overview of 501353IndexINICE 5ICH-I 119Immunocytochemistry 100Immunohistochemistry 100INT 52Interleukin converting enzyme (ICE) 5In situ Cell Death Detection Kit-AP 29-POD 29-Fluorescein 27In situ Cell Proliferation Kit-AP 90-FLUOS 88In situ nick translation 25ISNT method 25NADH/NADPH 113Natural killer cells 2, 50NBT 30Necrosisdefinition of 2difference between apoptosis and 3difference between cytotoxicity and 50inducers of 3inflammation during 3overview of 3secondary 3NEDD 117NF-kappa B 117Nick translation 25Nonradioactive assaysfor apoptosis 131for cell proliferation 132for DNA fragmentation 131Nucleosome quantification ELISA 22JJAM test 22KKi-67 98Ki-S5 98LLactate dehydrogenase, see LDHLDHCytotoxicity Detection Kit 52release assay 60Leakage/damage of plasma membrane,assays for 36LMW DNA 10Lymphokine-activated killer cells 50MAppendixMch2, Mch3, Mch4 119MCL-1 115Membrane symmetry during apoptosis 31Method selection guidefor apoptosis assay 7for cell proliferation assay 68Microwave pretreatment for TUNEL 108Mononucleosomes 9MORT-1 117M-phase 66MTP, see Microtiter plateMTTassay kit 73biochemical basis for reduction of 70cellular basis for reduction of 70comparison with other tetrazoliumsalts 72effect of superoxide dismutase on 113structure of 71use in cell proliferation assay 70use in cytotoxicity assay 583136OOligonucleosomes 9Pp53antibodies to 46pan ELISA 48significance of 46PARP 8PCNA 98Peripheral blood lymphocytesproliferation of 83stimulation of 83Phagocytic cells 3Phosphatidylserine 31Phospholipid 31Phospholipid-binding protein,see Annexin V 31Plasma membranedamage during apoptosis 3damage during necrosis 3Poly-(ADP-ribose) polymerase,see PARPPositive, false, TUNEL 105Proliferating cell nuclear antigen, seePCNAProliferating cellsassays for 73–75increased metabolic activity in 70Propidium iodideexclusion assay 36, 38properties 36Proteases in apoptosis 16Proteinase K pretreatment forTUNEL 108Proto-oncogene 44Indexlabel 30low labeling in 108nonspecific labeling in 107no signal in 108optimization of 107overview of 24POD 30pretreatments for 108protocol for tissues which tend to givefalse positives 105single reagents for 30special applications of 111specificity of 25tips for avoiding or eliminatingpotential artifacts in 107QQuestions frequently asked about celldeath assays 104RRadioactive assaysfor apoptosis 22for cell proliferation 84for DNA fragmentation 22Ras 118Reduced metabolic activity, assay for 58RIP 118STTdR proliferation assay 84TdT 25Terminal deoxynucleotidyl transferase 25Tetrazolium saltSee also MTT, WST-1, XTTmitochondrial reduction anduse in cell proliferation assays 70Thymidine release assay, radioactive 60Topoisomerase 98Topoisomerase inhibitor, see CampothecinTRADD 118Transferase, terminal 25Trypan blue exclusion assay 38, 67Two-color assay for dead and viableapoptotic cells 111TUNELAP 30definition of 25diminished staining during DNAcounterstaining 108dilution buffer 30effect of different fixatives on 107effect of pretreatments on 108enzyme 30evaluation of, for in situ apoptotic cellidentification 105false positives in 105high background in 107improvement of, for in situ apoptoticcell identification 105kits for 27–29UUptake of dyes by dead cells 37VViable cell number 67WWater-insoluble formazan 72Water-soluble formazan 72WST-1assay 75biochemical basis for reduction of 70cellular basis for reduction of 70comparison with other tetrazoliumsalts 72effect of reducing agents on 113effect of superoxide dismutase on 113structure of 71use in cell proliferation assay 70use in cytotoxicity assay 58XXTTassay kit 74biochemical basis for reduction of 70cellular basis for reduction of 70comparison with other tetrazoliumsalts 72effect of reducing agents on 113effect of superoxide dismutase on 113structure of 71use in cell proliferation assay 70use in cytotoxicity assay 58YZYAMA 119AppendixSpermatogonia, proliferating 91S-phase 66Staining of DNA 38Storage of samples for apoptosis assay 104Streptavidin conjugates 35“Sub-G1” peak 36Succinate-tetrazolium reductase 70Surface glycoproteins 31Symmetry of membranes duringapoptosis 311373List of International RepresentativesAppendixList of International Representatives3AustraliaRoche Diagnostics Australia Pty.